I.U.B.:1.4.3.2
L-AminoAcidOxidaseisanoligomericglycoproteincomposedofunequalamountsoftwodifferentapproximately70kDasubunits.Threeelectrophoreticallydifferentisozymesoccurasdifferentcombinationsofthetwosubunits.thereareapproximatelytwomolesofFADpermoleofholo-enzyme.L-aminoacidoxidasecatalyzestheoxidativedeaminationofanumberofL-aminoacids.TheenzymeisabsolutelyspecificforL-isomers.TheWorthingtonproductispreparedaccordingtoWellnerandMeister,JBC,235,2013,(1960)tothepointjustpriortocrystallization.
StABIlity/Storage:Theenzymeisstableinsolutionfor6-12monthsat2-8°C.Thepresenceofsubstrateandtheabsenceofofoxygenstabilizetheenzymeatelevatedtemperatures.TheenzymemaybereversIBLyinactivatedbyincubationat38°Cinphosphatebuffer,pH7.5(Wellner,Biochem.,5,1586,1966).Curti,etal.,JBC,243,2306,1968reportreversibleinactivationuponfreezing.Storeat2-8°C.Donotfreeze.
UnitDefintion:OneUnitoxidizesonemicormoleofL-leucineperminuteat25°C,pH7.6
