Acompletemethoddevelopmentkitcontaininganassortmentofenzymesmostfrequentlyusedinenzymatictissuedissociationandcellisolationprocedures.Includesinstructions,referencesandstrategiesforthehandling,useandoptimizationofenzymaticcellisolationmethodsformaximumyieldofviablecells.Containsallenzymescommonlyreferencedintissuedissociationandcellisolationproceduresandthe CellIsolationGuide describingthetissuetypescommonlyused,themodeofactionofthevariousenzymes,tissueculturetechniques,andprotocoloptimizationguidelines,withcell-andtissue-specificreferencesforgettingstartedinenzymaticcellisolation.
TissuedissociationandcellharvestingaretwoprincipalapplicationsforenzymesintissuecultureresearchandcellBIOLOGystudies.Despitethewidespreaduseofenzymesfortheseapplicationsovertheyears,theirmechanismsofactionindissociationandharvestingarenotwellunderstood.Asaresult,thechoiceofonetechniqueoveranotherisoftenarbitraryandbasedmoreonpastexperiencethanonanunderstandingofwhythemethodworksandwhatmodificationscouldleadtoevenbetterresults.
Researcherssearchingthescientificliteratureforinformationontheidealenzymesandoptimalconditionsfortissuedissociationareoftenconfrontedwithconflictingdata.Muchofthevariationstemsfromthecomplexanddynamicnatureoftheextracellularmatrixandfromthehistoricaluseofrelativelycrude,undefinedenzymepreparationsforcellisolationapplications.Theextracellularmatrixiscomposedofawidevarietyofproteins,glycoproteins,lipidsandglycolipids,allofwhichcandifferinabundancefromspeciestospecies,tissuetotissueandwithdevelopmentalage.TheWorthingtonCellIsolationOptimizingSystemprovidesanassortmentofthewidelyusedenzymesinpurifiedformforestablishinganoptimizedcellisolationprocedureonacost-efficientbasis.
KitContents
•CollagenaseType1CLS1500mgdw•HyaluronidaseHSE50,000un
•CollagenaseType2CLS2500mgdw•ElastaseESL100mgP
•CollagenaseType3CLS3500mgdw•PapainPAPL100mgP
•CollagenaseType4CLS4500mgdw•DeoxyribonucleaseIDP25mgdw
•TrypsinTL500mgdw•TrypsinInhibitorSIC100mgdw
•NeutralProtease(Dispase)NPRO10mgdw
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