I.U.B.:3.1.21.1 

BovinepancreaticdeoxyribonucleaseIproducedrecombinantlyinyeast,Pichiapastoris,todecreaselevelsofcontaminatingRNaseandeliminatepotentialpathogensassociatedwithanimalbasedmaterials.

BovinepancreasisarichsourceofRNaseAwhichisoftenfoundinmanycommercialDNasepreparations.ProducingDNaseIbyrecombinantmeansinanorganismwithmuchlowerlevelsofendogenousRNasegreatlyfacilitatespurificationofanenzymewithundetectablelevelsofRNase.TheprocessesinvolvedintheproductionandisolationofrecombinantDNaseIarecompletelydevoidofanimalbasedcomponentswhicheliminatesthepossibilityofintroducinganimalderivedpathogensintobioprocessingprocedures.

RecombinantDNaseIissuitableforsuchapplicationsas:

•RemovinggenomicDNAfromRNApreparationspriortoRT-PCR

•DegradationofDNAtemplatesaftertranscriptionreactions

•RemovingunwantedDNAfromsamplespriortoNorthernblotting

•RemovingDNAduringbiopharmaandbioprocessingprocedures

UnitDefinition:OneUnitcausesanincreaseinabsorbanceat260nmof0.001perminuteat25^oCwhenactinguponhighlypolymerizedDNAatpH5.0,whichisthesameasotherWorthingtonDNaseIproducts.

Note:Kunitzunitsasreportedbyothersupplierscanbe2to4timeshigherthanKunitzunitsasmeasuredatWorthington.AsmeasuredatWorthington,OneKunitzunitdigests1mgofcalfthymus(orpUC19orl-phage)DNAin10minutesat37^oCin50mMTris,1mMMg²⁺,1mMCa²⁺,pH7.8.CorrelationofdigestionunitswithKunitzunitsmaybedifferentinotherbuffersystems.

StorageBuffer(DR1S):5mMcalciumacetate,4mg/mlglycine,pH5.0and50%glycerol.

DNaseIReactionBuffer(10X):500mMTris-HCl,10mMMgSO₄,1mMCaCl₂,pH7.8,provided.

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