WorthingtonoffersDNApurifiedfromthesesources:

Calfthymus:(Code:DNA)PreparedandpurifiedbyamethoddevelopedatWorthingtontohavelowerproteinandRNAcontaminationthanmostothercommercialpreparations.ThishighlypolymerizedDNAisanexcellentsubstratefordeoxyribonuclease.Asodiumsalt,itmustbeconvertedbyaddingmagnesiumionstobesusceptIBLetoDNase.

CalfthymusDNA,covalentlyboundtocelluloseisalsoavailable:
DNACellulose,Double-Stranded(Code:DNACELDS)
DNACellulose,Single-Stranded(Code:DNACELSS)

SalmonTestes:(Code:SDNA)PreparedbyamodificationofthemethodofEmanuelandChaikoff,J.Biol.Chem.,203,164,(1953).Aminimumof75%nativenucleicacid.

SalmonTestesDNA,Denatured&Fragmented(Code:SDNAD)isalsoavailable.Preparedbymechanicalshearingandheatdenaturation.

Escherichiacoli:(Code:DNAEC)IsolatedasdescribedbyMarmur,J.Mol.Biol.,3,208,1961.

LamBDaphageDNA(Code:DNAL)ispreparedfromCsClpurifiedphageandispurifiedtoanA₂₆₀/A₂₈₀≥1.8.Homogeneousinagarosegelelectrophoresis.Asolutionin10mMTris-HClpH8.0with1mMEDTA.

DNAfragmentspreparedbyrestrictionendonucleasedigestionofpurifiedlambdaDNAareavailable(Codes:DNALBSTE;DNALECOR;DNALHIND).Suppliedassolutionsin10mMTris-HClpH8.0with1mMEDTA.

TechnicalNote:OneA₂₆₀unit=50µgDNA.

StABIlity/Storage:DNAL:Storagebuffer10mMTris-HCl,pH8.0containing1mMEDTA.Storeat-20°C. Oncethawedkeepat2-8°C.

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