I.U.B.:3.4.21.36
Porcinepancreaticelastasehasamolecularweightof25.9kDa,andapHoptimumof8.5. Whileelastasewillhydrolyzeawidevarietyofproteinsubstrates,itisuniqueamongproteasesinitsABIlitytohydrolyzenativeelastin,asubstratenotattackedbytrypsin,chymotrypsinorpepsin.Soybeantrypsininhibitorandkallikreininhibitorsuppressproteolyticbutnotelastolyticactivity. ElastaseisassayedusingamethodadaptedfromthatofFeinsteinetal.,Biochem.Biophys.Res.Comm.,50,1020(1973)andusingthemoresolublesubstrateofBiethetal.,Biochem.Med.,11,350(1974).
Stability/Storage:ElastaseisunstableatpH≤3.5.Whenstoredasadrypowdertheenzymeisstablefor6-12monthsat2-8°C. Elastaseproductcodes:ESandESLhavepoorsolubilityatneutralpHandatconcentrationsgreaterthan0.25%. ItishelpfultomakeprimarysolutionsinKCloralkalinebuffersbeforedilutingintothereactionmixturesormedia,compensatingforionicstrengthorpHchanges. StableatpH4.0-10.4.
TechnicalNotes:1SucAla3NAunitisapproximatelyequivalentto6elastindigestionunits.AqueousliquidsUSPensionsshouldbeasepticallyhandledtoavoidbacterialcontamination.Duetotheviscousnatureoftheaqueoussuspension(Code:ES)thevialshouldberinsedtorecovercontents.
UnitDefinition:OneUnitcleavesonemicromoleofN-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilideperminuteat25°C,pH8.0.
RelatedProducts:
- Collagenase(CLS1-4/CLSPA)
- DeoxyribonucleaseI(DP/D/DCLS/D2/DPFF/DPRF)
- Hyaluronidase(HSE/HSEP)
- NeutralProtease(Dispase,NPRO)
- Papain(PAP/PAPL/PAP2)
- Pepsin(PM)
- ProteinaseK(PROK)
- Trypsin(TL/TRL/TRL3/TRLS/TRTPCK)
- TrypsinInhibitors(LBI/OI/SI/SIC)
- CellIsolationOptimizimgSystem(CIT)
- HepatocyteIsolationSystem(HIS)
- NeonatalCardiomyocyteIsolationSystem(NCIS)
- PapainDissociationSystem(PDS/PDS2)
