I.U.B.:3.2.1.35

Testicularhyaluronidaseisaglycoproteincontaining5%mannoseand2.7%glucosamine.OptimumpHrangeis4.5-6.0.Theenzymecatalyzesthehydrolysisofendo-N-acetylhexosaminicbondsofhyaluronicacidandchondroitinsulfateAandC(butnotB),primarilytotetrasaccharideresidues.

UnitDefinition:Oneunitisbasedonthechangeinabsorbency(turbidity)at540nmofaninternalstandardassayedconcurrentlywitheachlot. InternalstandardreplacesUSP/NFreferencenolongeravailable.

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