I.U.B.:3.2.1.17

Lysozymepreferentiallyhydrolyzestheβ-1,4glycosidiclinkagesbetweenN-acetylmuramicacidandN-acetylglucosaminewhichoccurinthemucopeptidecellwallstructureofcertainmicroorganisms,suchasMicrococcuslysodeikticus(Productcode:ML).Aslightlymorelimitedactivityisexhibitedtowardchitinoligomers.Ithasamolecularweightof14,388daltons.OptimumpHis9.2.Lysozymeisinhibitedbysurfaceactiveagentssuchasdodecylsulfate,alcoholandfattyacids.Imidazoleandindolederivativesforminhibitorychargedtransfercomplexes.

StABIlity/Storage:Stablefor3-5yearsat2-8°C.SolutionsatpH4-5arestableforseveralweeksrefrigeratedandfordaysatambienttemperatures.Storeat2-8°C.

TechnicalNote:Duetoassaydifferences,8,000u/mgbyWorthington"sassayisequivalentto50,000u/mgclaimedbyothersuppliers.

UnitDefinition:OneUnitisequaltoadecreaseinturbidityof0.001perminuteat450nmatpH7.0and25°C,usinga0.3mg/mlsUSPensionofMicrococcuslysodeikticuscells(WBCproductcodeML)assubstrate.

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