I.U.B.:3.4.24.28
Neutralprotease(Dispase®)isanon-mammaliananimaloriginfree(AOF)metallo,neutralprotease,purifiedbymethodsdevelopedatWorthington.Itsmildproteolyticactionmakestheenzymeespeciallysuitableforthepreparationofprimarycellsandsecondary(subcultivation)cellculture,sinceitisgentleoncellmembranes.Thisproteaseisalsousedasasecondaryenzymeincellisolationandtissuedissociationapplications,commonlyusedwithcollagense.
CharacteristicsofNeutralProtease(Dispase®)fromBacilluspolymyxa:
MolecularWeight: 36kda.
pHOptimum: StableoverawidepHrange:4.0-9.0,optimumpH5.9-7.0.
StABIlity/Storage:Stableat2-8°Cfor12months.Aliquotandstoreat-20°Cafterreconstitutionwithwaterorcommonlyusedbalancedsaltsolutionsormedia.
UnitDefinition:OneUnitreleasesonemicromoleofFolinpositiveaminoacids,measuredastyrosine,at37°C,pH7.5,usingcaseinasthesubstrate.
Specificity:Non-specificcleavageofpeptidebondscontainingleucineandphenylalanine.
Activators:DivalentcationsincludingCa2+,Mg2+,Mn2+,andFe2+
Inhibitors:EDTA(1mM),EGTA,1-10-phenanthrolineandheavymetals
Application(s):Commonlyusedtoseparateskinepidermisfromdermisleavingintactepithelialsheetsandstemcell,hepatocyteandothercellisolationapplications.However,duetothediversityofthevariablesinvolved,exactisolationconditionsshouldbedeterminedempiricallyforeachcell/tissueapplication.
RelatedProducts:
Collagenase(CLS1-4/CLSS1-4/CLSPA)
DeoxyribonucleaseI(DP/D/DCLS/D2/DPFF/DPRF)
Elastase(ES/ESL/ESFF)
Hyaluronidase(HSE/HSEP)
Papain(PAP/PAPL/PAP2)
ProteinaseK(PROK)
Trypsin(TL/TRL/TRL3/TRLS/TRTPCK)
TrypsinInhibitors(LBI/OI/SI/SIC)
CellIsolationOptimizingSystem(CIT)
HepatocyteIsolationSystem(HIS)
NeonatalCardiomyocyteIsolationSystem(NCIS)
PapainDissociiationSystem(PDS/PDS2)
