Source: Aspergillus oryzae

I.U.B.: 3.1.30.1

Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn²⁺ and/or Ca²⁺. Inhibitors are EDTA, citrate and high concentrations of SDS.

Stability/Storage: For long term storage in solution, for up to six months, dilute NUCSI to ≥6000 u/ml in water and freeze in aliquots. Dilute solutions can be stabilized by adding 0.1% albumin (Worthington Code: BSANF) and 10% glycerol.

Unit Definition: One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.

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