I.U.B.:3.4.22.2

PapainisasulfhydrylproteasefromCaricapapayalatex.Ithasamolecularweightof23kDaandanoptimumpHrangeof6.0-7.0. Theactionofpapainonleucinemethylesterproducesaninsolublepolyleucinepeptide.Papainbreaksdowntheintercellularmatrixofcartilage.Papainisactivatedbycysteine,sulfide,andsulfite.StABIlizingagentsareEDTA,cysteineanddimercaptoethanol.

Stability/Storage:Stablefor6-12monthsat2-8°C. Donotfreezeaqueoussuspensions.

TechnicalNotes:Papainpreparationsshouldbeincubatedintheactivationsolutionbeforeusetoensurefullactivity.Applicationsincludeantibodyfragmentationandprimary/neuralcellisolation.

UnitDefinition:OneUnithydrolyzesonemicromoleofbenzoyl-L-arginineethylesterperminuteat25°C,pH6.2,afteractivationinasolutioncontaining1.1mMEDTA,0.067mMmercaptoethanoland5.5mMcysteine-HClfor30minutes.

RelatedProducts:

Collagenase(CLS1-4/CLSPA)
DeoxyribonucleaseI(DP/D/DCLS/D2/DPFF/DPRF)
Hyaluronidase(HSE/HSEP)
NeutralProtease(Dispase,NPRO)
ProteinaseK(PROK)
Trypsin(TL/TRL/TRL3/TRLS/TRTPCK)
TrypsinInhibitors(LBI/OI/SI/SIC)

CellIsolationOptimizimgSystem(CIT)
HepatocyteIsolationSystem(HIS)
NeonatalCardiomyocyteIsolationSystem(NCIS)
PapainDissociiationSystem(PDS/PDS2)