I.U.B.:4.2.2.10

Purifiedpectinaseisamulti-componentpreparationhighlyeffectiveindepolymerizingplantpectinswithvaryingdegreesofesterification.Theproductcontainssubstantialhemicellulase,cellulase,pectinesteraseandxylanaseactivitieswhichtogetherwithpectinlyaseandpolygalacturonaseworksynergisticallytodigestplantcellwalltissues.WhenusedwithWorthingtonpurifiedcellulase,purifiedpectinasehasbeenfoundtobehighlysuccessfulforgeneratinggoodyieldsofviableprotoplastsinseveralplantsystems,e.g.,corn,soybean,redbeet,sunflower,tomatoandcitrus.Ingeneral,aconcentrationrangeof0.1%to0.5%pectinase(withaccompanying0.5%to1.5%cellulase)usedat24°Cto37°Cforperiodsof1to16hourswillyieldgoodresults.

StABIlity/Storage:Protectfrommoisture.Ifnotusingentirebottleatonce,weighintosingle-usealiquotsonarrivalandstoretightlycoveredanddessicated,at2-8°C.Materialisveryhygroscopicandcanbecometackyanddifficulttoweighifexposedtomoisture.

UnitDefinition:OneUnitreleases1µmoleofD-galacturonicacidfrompolygalacturonicacidperminuteat37°C,pH5.0.

TechnicalNote:Pectinaseisextremelyhydroscopic;storedessicatedtoprotectfrommoisture.

RelatedProducts:Cellulase(CEL/CELF)