Source:BovinePlasma

I.U.B.:1.4.3.21

Plasmaamineoxidase(PAO)catalyzesthereaction:

RCH₂NH₂+O₂+H₂O→RCHO+NH₃+H₂O₂

Bovineplasmaamineoxidasehasamolecularweightof170kDaandanoptimumpHof6.2forspermineand7.2forspermidine.Amineoxidasesaredividedintotwoclasses:thepyridoxalandcoppercontainingenzymetowhichplasmaamineoxidasebelongs,andtheFAD-containingaminooxidases.Naturalsubstratesincludecatecholamines,tryptaminederivativesandotherphysiologicallyactiveamines.Plasmaamineoxidaseisusedinresearchrequiringnitrogengrouptransfers.Themoleculeiscomposedoftwoidenticalpolypeptidechains.TherearetwopyridoxalphosphatesandtwoatomsofCu⁺permolecule.Bovineplasmaamineoxidaseisinhibitedbycopperchelatingagents,manycarboxylreagentssuchascuprizone,hydroxylamineandcyanide.Benzoicacidandbenzylalcoholarebothnon-competitiveinhibitors(K_I=30and34mMrespectively).TheassayfordeterminationofamineoxidasesemployedatWorthingtonisessentiallythatofTaboretal.,JBC,208,645,(1954)withthereactiontemperaturereducedto25°C.

StABIlity/Storage:Stablefor12monthsat-20°C.Storeat-20°C.

UnitDefinition:1internationalunitoxidizes1µmoleofbenzylamineperminuteat25°C,pH7.2.

TechnicalNote:1I.U.equals4,330Taborunits.(T.U.)