I.U.B.:3.4.21.19

ProteaseS.aureusV8(Endoproteinase-Glu-C)specificallycleavespeptidebondsontheCOOH-terminalsideofeitherasparticorglutamicacids.Inthepresenceofammonium,theenzymespecificityislimitedtoglutamicsites.Ithasamolecularweightof27,000daltonsandoptimumpH"sof4.0and7.8withhemoglobinasthesubstrate.ProteaseS.aureusV8isinhibitedbydiisopropylfluorophosphateandmonovalentanionssuchasF^-,Cl^-,CH₃COO^-andNO³.EnzymeactivityisdeterminedbythecaseindigestionassaydescribedbyDrapeau(MethodsEnzymol.,45,469,1976).

StABIlity/Storage:Autolysisoccursattemperaturesgreaterthan40°C.TheenzymeisfullyactiveinUSP0.2%SDS.Stablefor12monthsat2-8°C.

UnitDefinition:OneUnitcausesachangeof0.001A₂₈₀nmperminuteat37°C,pH7.8usingcaseinasthesubstrate.

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