I.U.B.:3.4.21.64

ProteinaseK(PROK)isaserineproteasewithbroadspecificitytowardsaliphatic,aromaticandotherhydrophobicaminoacids.PROKhasamolecularweightof27,000daltonsandisCa²⁺dependent.ItisnotinactivatedbymetalionchelatingagentssuchasEDTA,sulfhydrylreagents,PCMB,TLCK,orTPCK.Italsoretainsactivityin0.5%SDS.ItcanbeinhibitedbyPMSForDFP.

StABIlity/Storage:Thelyophilizedpowderisstableforoneyearat2-8°C.Solutionsin50mMTris-HCl,pH8.0with1mMCaCl₂arestableformonthsat2-8°C.Storeat2-8°C.

UnitDefinition:OneUnitreleasesonemicromoleofFolinpositiveaminoacids,measuredastyrosine,at37°C,pH7.5,usingureadenaturedhemoglobinasthesubstrate.

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