I.U.B.:2.7.7.49

Chromatographicallypurifiedheterodimercomposedof66kDaand51kDasubunits.Suppliedasasolutionin10mMpotassiumphosphate,pH7.4,1mMDTTand20%glycerol.PrimarilyforAIDSresearchpurposes;thisenzymehaslessfidelitythantheAMVenzymeinotherapplicationssuchasthepreparationofCDNAfrommRNAforcloningpurposes.

UnitDefinition:OneUnitincorporates1nmoleoftritiatedd-TMPintoacidprecipitableproductsusingpoly(A)/oligo(dT)12-18asthetemplate/primerin20minutesat37°C,pH8.3.

Note:Thecurrentassayusesamoreefficienttemplate/primercombinationthatresultsinanapproximatetwo-foldincreaseinspecificactivitycomparedtoourpreviousassay.Asaresult,Worthington"spackagesizescontainthesameamountofproteinwithtwiceasmuchactivitybaseduponthenewassay.

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