I.U.B.:3.4.21.4
Trypsinisapancreaticserineproteasewithsubstratespecificitybaseduponpositivelychargedlysineandargininesidechains.Itisderivedfroma34kDainactiveprecursorzymogen,trypsinogen,afterenzymaticremovalofanN-terminal6-aminoacidleadersequenceresultinginthe23.8kDatrypsinmolecule.TheoptimumpHis8.0.Trypsinisinhibitedbyorganophosphoruscompoundssuchasdiisopropylfluorophosphateandnaturalinhibitorsfrompancreas.Soybean,limabean,andeggwhitearealsosourcesofnaturalinhibitors.TrypsincleavesamideandesterbondsofArgandLys.TheWorthingtonSequencingGradeTrypsinhasbeenfurtherpurifiedtoremovetracecontaminatingproteasesandautolysisproductswhichcouldinterfereintrypsindigestionexperiments,andexhibitsasinglebandonPAGE.
Uses:Fortissueculturework,Worthingtontrypsin,Codes:TRL,TRLS,TRLVMFandTRTVMFhavebeenusedbymanyresearchers.ProductCodes:TRSEQZ,TRSEQIIandTRTPCKaretypicallyusedforproteinsequencing,mappingandstructurestudies.Worthingtonmodifiedsequencinggradetrypsin,ProductCode:TRSEQZ,issubjectedtoextensivepurificationtoremovecontaminatingproteasesandtrypticautolysisby-productswhichcouldaffectthespecificityofthedigestionprocess. Subsequently,theenzymeischemicallymodifiedtominimizetheautolysisprocessaswellasincreasethestability.Themodifiedtrypsinisprocessedfurthertoremoveresidualautodegradationproducts.Thespecificityoftheenzymeisroutinelycheckedafterthechemicalmodification.
Stability/Storage:MostgradesofWorthingtontrypsinarestablefor2-3yearswhenstoredat2-8°C.Protectfrommoisture.
UnitDefinitions:TAMEUnit:OneUnithydrolyzes1µmoleofp-toluene-sulfonyl-L-argininemethylester(TAME)perminuteat25°C,pH8.2,inthepresenceof10mMcalcium.
Note:1mgtrypsin≥180TAMEunits,10,350BAEEunits,3,450USP/NFunits.
TechnicalNotes:TheVirusandMycoplasmaFreetrypsin(Code:TRTVMF)hasbeenfilteredthroughan0.22micronporesizemembrane,lyophilized,subjectedtogammairrADIation,andtestedforvirusandmycoplasma.
RelatedProducts:
- Chymotrypsin(CDAG/CDS/CDI)
- Collagenase(CLS1-4/CLSPA)
- DeoxyribonucleaseI(DP/D/DCLS/D2/DPFF/DPRF)
- Hyaluronidase(HSE/HSEP)
- NeutralProtease(Dispase,NPRO)
- ProteinaseK(PROK)
- TrypsinInhibitors(LBI/OI/SI/SIC)
- CellIsolationOptimizimgSystem(CIT)
- HepatocyteIsolationSystem(HIS)
- NeonatalCardiomyocyteIsolationSystem(NCIS)
- PapainDissociationSystem(PDS/PDS2)
