LimaBeanInhibitor:Limabeantrypsininhibitorwhichinhibitsbovineaswellashumantrypsinandplasmin,actsuponbothtrypsinandchymotrypsinbyformingequimolarcomplexes.Limabeaninhibitorsmaybechromatographicallyseparatedintoasmanyassixvariants.Jonesetal.,Biochem.,2,66,(1963)characterizedfourofthem.Allhavesimilarbutnotidenticalaminoacidcomposition,containsixorsevendisulfidebondsandlackmethionineandtryptophan.Molecularweightsvarybetween8kDaand10kDa.
StABIlity/Storage:Thelimabeaninhibitorisstable1-2yearsat2-8°C.

Ovomucoid:Ovomucoidsaretheglycoproteinproteaseinhibitorsofavianeggwhite.Thereareseveralproteaseinhibitorsineggwhite.TheWorthingtonproductisthatdescribedbyLineweaverandMurrayJBC,171,565(1947).Ithasamolecularweightofapproximately28kDa.
Stability/Storage:Ovomucoidisstable1-2yearswhenstoredat2-8°C.

PancreaticInhibitor:ThepancreaticinhibitorproducedbyWorthingtonisoftenreferredtoastheKunitzinhibitorandwasfirstcrystallizedbyKunitzandNorthropJ.Gen.Physiol.,19,991(1936).Itformsaverystable1:1complexwithbovinetrypsinbetweenpH3and10andalsowithhumantrypsins.Chymotrypsinisalsoinhibitedbythisproductbutisboundlesstightly.Theesterolytic,proteolyticandelastolyticactivitiesofporcineelastase,ontheotherhand,arenotinhibited.TheKunitzinhibitorisasinglepolypeptidechainof58aminoacidsandhasamolecularweightof6.5kDa.Whenlungtissueisusedasthesource,theinhibitoriscalledaprotinin.
Stability/Storage:Thepancreaticinhibitorisstable1-2yearswhenstoredat2-8°C.

SoybeanInhibitor:ThesoybeantrypsininhibitorwasfirstcrystallizedbyKunitzin1945andisoneofseveralsuchinhibitorsfoundinsoybeans.Itsmolecularweightis21,500±800daltonsandtheoptimumpHis7.0.Soybeaninhibitorinhibitstrypsinmole-for-moleandtoalesserextentchymotrypsin.
Stability/Storage:Thesoybeaninhibitorisstablefor1-2yearsat2-8°C.

UnitDefinition:Theactivityoftheinhibitorsisexpressedastheamountoftwicecrystallizedtrypsin(WorthingtonCode:TRL)inhibitedpermilligramofinhibitor.1mgTRL≥180TAMEunits,10,350BAEEunits,3,450USP/NFunits.

RelatedProducts:

Chymotrypsin(CDAG/CDS/CDI)
Collagenase(CLS1-4/CLSPA)
DeoxyribonucleaseI(DP/D/DCLS/D2/DPFF/DPRF)
Hyaluronidase(HSE/HSEP)
NeutralProtease(Dispase,NPRO)
Papain(PAP/PAPL/PAP2)
ProteinaseK(PROK)
Trypsin(TL/TRL/TRL3/TRLS/TRTPCK)

CellIsolationOptimizimgSystem(CIT)
HepatocyteIsolationSystem(HIS)
NeonatalCardiomyocyteIsolationSystem(NCIS)
PapainDissociationSystem(PDS/PDS2)