I.U.B.: 3.1.3.1

Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.

Source/Unit Definitons:CAP: Calf intestine. One Worthington Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 37°C, pH 9.8.

BAPF, BAPC, BAPSF: E. coli. One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.

PC: Chicken Intestine. One Unit hydrolyzes 1µmole of o-carboxyphenol phosphate per minute at 25°C, pH 8.8.

Technical Notes: Worthington chicken intestine alkaline phosphatase (Code: PC) is the preparation used in the NF/USP dexamethasone phosphate measurement.

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